Stages of Mitosis.

Overview
For this project, we were tasked with creating a visual representation of a biological process using the equipment available in the lab.
 
After a brainstorming session, we decided to make a HUE animation of the process of mitosis. For this project, we decided to use the 3D printers, the laser cutter, a white board, manila papers and marker pens.
 
We used 123D design to design the shape of the chromatids.  We used a series of stretched out spheres and joints (ball and socket) to create interlocking chromatids.

We then used both the AFINIA and the BUKITO 3D printers to print our models.  You could also use clay to model the chromosomes, as this saves both time and energy.
 
Since the 3D printer couldn’t print a good quality model of the centrioles, we modeled them using clay.
 
We also laser cut letters to use in the animation to show the different stages of mitosis. Here you could also cut out letters from manila paper for the same function.
 
After all out props were ready, we used markers to draw a cell on a whiteboard and started filming the process of mitosis using HUE animation.  Later, we added the soundtrack to the motion picture animation and made some edits to come up with the final video.
 
Challenges encountered:
- The 3D printer is so slow. To save on time, we suggest using clay.
- Glare on the whiteboard. Eliminate any overhead light sources that shine light directly onto the white board.
 
Authors.

Project by:
Luke Hanley, Vincent Bett, and Nico Lusardo.

Finished Animation Video

Mitosis is the cellular process of nuclear division that includes cytokinesis, resulting on the production of two identical daughter cells 

The phases of mitosis include:

Interphase: a relatively short period of the cell cycle where the cell prepares itself for the process of cell division by copying its DNA in preparation for mitosis.
 
Prophase: the cell continues to prepare for division by condensing its recently duplicated chromatin into chromosomes; these copies are known as sister chromatids and are attached to each other at the centromere.  Centrioles begin migrating to opposite ends of the cell nucleus during this stage. 
 
Prometaphase: the nuclear membrane begins to disintegrate as the chromosomes move towards the center of the cell to attach to spindle fibers (specialized tubules).
 
Metaphase: now all of the pieces are aligning themselves for the "big split." The chromosomes line up along an imaginary central plane called the metaphase plate and the centrioles send out spindle fibers that connect to the chromosomes. The tubules actually connect to the centromere. 
 
Anaphase: Here we go! The separation begins. Half of the chromosomes are pulled to one side of the cell; half go the other way. When the chromosomes get to the side of the cell, it's time to move on to telophase. 
 
Telophase: Now the division is finishing up. This is the time when the cell membrane closes in and splits the cell into two pieces. You have two separate cells each with half the number of the original chromosomes
 
Cytokinesis divides the cytoplasm of the parental cell into two daughter cells.

(The soundtrack was downloaded from freemusicarchive.org)

Initial Project Planning on White Board
Chromosome Design Process
Chromosome 3D Printing
Chromosome Assembly
Centrosome 3D Design Process
Clay Designs for Chromosomes and Centrosomes
Letters for Phase Titles
Materials Ready for Animation

 WIth all our materials contructed, we decied to use the 3D printed chromosomes and the clay centrosomes in the final video. We also cut string as a possible material for the spindle fibers, but we ended up using dry-erase markers, at they were easier to animate with. We used paper circles for the nuclei and laser-cut wooden letters for the titles.

Animation Station Setup
Animation Process

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